Engineering the T6SS of Pseudomonas for targeted delivery of antibacterial and antifungal effectors

Author(s): Alicia Isabel Pérez-Lorente, Mario Araujo-Garrido, Antonio de Vicente, Diego Romero and Carlos Molina-Santiago
Source: Journal of Biological Engineering  Volume: 19 Published: 2025
DOI: https://doi.org/10.1186/s13036-025-00497-w

Diversification of Lipopeptide Analogues Drives Versatility in Biological Activities

Author(s): Montserrat Grifé-Ruiz, Jesús Hierrezuelo-León, Antonio de Vicente, Alejandro Pérez García and Diego Romero
Source: Journal of Agricultural and Food Chemistry  Volume: 73, 2 Published: 2025
DOI: https://doi.org/10.1021/acs.jafc.4c11372

Unravelling the impact of environmental factors in shaping plant microbiomes

Author(s): Alicia Isabel Pérez-Lorente, Diego Romero, Carlos Molina-Santiago
Source: Microbial Biotechnology  Volume: 7, 6 Published: 2024
DOI: https://doi.org/10.1111/1751-7915.14504

Cyclo(Pro-Tyr) elicits conserved cellular damage in fungi by targeting the [H⁺]ATPase Pma1 in plasma membrane domains

Author(s): D. Vela-Corcia, J. Hierrezuelo, A.I. Pérez-Lorente, P. Stincone, A. K. Pakkir Shah, A. Grélard, Y. Zi-Long, A. de Vicente, A. Pérez-García, L. Bai, A. Loquet, D. Petras & D. Romero
Source: Communications Biology  Volume: 7, 1253 Published: 2024
DOI: https://doi.org/10.1038/s42003-024-06947-3

microbeMASST: a taxonomically informed mass spectrometry search tool for microbial metabolomics data

Author(s): Zuffa, S., Schmid, R., Bauermeister, A., … , Pérez-Lorente, A.I., Molina-Santiago, C., Romero, D. et al.
Source: Nature Microbiology  Volume: 9, pages 336–345 Published: 2024
DOI: https://doi.org/10.1038/s41564-023-01575-9

Incorporation of bioactive compounds from avocado by-products to ethyl cellulose-reinforced paper for food packaging applications

Author(s): Maria A. Acquavia, José J. Benítez, Giuliana Bianco, Maria A. Crescenzi, Jesús Hierrezuelo, Montserrat Grifé-Ruiz, Diego Romero, Susana Guzmán-Puyol, José A. Heredia-Guerrero
Source: Food Chemistry  Volume: 429 Published: 2023
DOI: https://doi.org/10.1016/j.foodchem.2023.136906

Digestive exophagy of biofilms by intestinal amoeba and its impact on stress tolerance and cytotoxicity

Author(s): Zanditenas, E., Trebicz-Geffen, M., Kolli, D., … , Domínguez-García, L., Romero, D., et al.
Source: npj Biofilms Microbiomes  Volume: 9, 77 Published: 2023
DOI: https://doi.org/10.1038/s41522-023-00444-x

Molecular characterization of the N-terminal half of TasA during amyloid-like assembly and its contribution to Bacillus subtilis biofilm formation

Author(s): Cámara-Almirón, J., Domínguez-García, L., El Mammeri, N., Romero, D., et al.
Source: npj Biofilms Microbiomes  Volume: 9, 68 Published: 2023
DOI: https://doi.org/10.1038/s41522-023-00437-w

Sporulation Activated via σ^W  Protects Bacillus from Tse1 Peptidoglycan Hydrolase Type VI Secretion System Effector

Author(s): A.I. Pérez-Lorente; C. Molina-Santiago; A. de Vicente; D. Romero
Source: Microbiology Spectrum Volume: 11-2 Published: 2023
DOI:10.1128/spectrum.01185-23

Native metabolomics identifies the rivulariapeptolide family of protease inhibitors.

Author(s): R. Reher; A.T. Aron; P. Fajtová; P. Stincone; B. Wagner; A.I. Pérez-Lorente; C. Liu; I.Y.B. Shalom; W. Bittremieux; M. Wang; K. Jeong; M.L. Matos-Hernandez; K.L. Alexander; E.J. Caro-Diaz; C.B. Naman; J.H.W. Scanlan; P.M.M. Hochban; W.E. Diederich; C. Molina-Santiago; D. Romero; K.A. Selim; P. Sass; H. Brötz-Oesterhelt; C.C. Hughes; P.C. Dorrestein; A.J. O’Donoghue; W.H. Gerwick; D. Petras
Source: Nature Communications Volume: 13 – 1 Published: 2022
DOI:10.1038/s41467-022-32016-6

Rational Design of Chitin Deacetylase Inhibitors for Sustainable Agricultural Use Based on Molecular Topology

Author(s): R. Zanni; J. Martínez-Cruz; M. Gálvez-Llompart; D. Fernández-Ortuño; D. Romero; R. García-Domènech; A. Pérez-García; J. Gálvez.
Source: Journal of Agricultural and Food Chemistry  Volume: 70 – 41, pp. 13118 – 13131 Published: 2022
DOI:1021/acs.jafc.2c02377

Suppression of Chitin-Triggered Immunity by a New Fungal Chitin-Binding Effector Resulting from Alternative Splicing of a Chitin Deacetylase Gene

Author(s): J.M. Martínez-Cruz; Á. Polonio; L. Ruiz-Jiménez; A. Vielba-Fernández; J. Hierrezuelo; D. Romero; A. de Vicente; D. Fernández-Ortuño; A. Pérez-García.
Source: Journal of Fungi  Volume: 8 – 10 Published: 2022
DOI:3390/jof8101022

A Noninvasive Method for Time-Lapse Imaging of Microbial Interactions and Colony Dynamics.

Author(s): C. Molina-Santiago; J.R. Pearson; M.V. Berlanga-Clavero; A.I. Pérez-Lorente; A. de Vicente; D. Romero.
Source: Microbiology Spectrum.  Volume: 10 – 4 Published: 2022
DOI:10.1128/spectrum.00939-22

Bacillus subtilis biofilm matrix components target seed oil bodies to promote growth and anti-fungal resistance in melon.

Author(s): M.V. Berlanga-Clavero; C. Molina-Santiago; A.M. Caraballo-Rodríguez; D. Petras; L. Díaz-Martínez; A. Pérez-García; A. de Vicente; V.J. Carrión; P.C. Dorrestein; D. Romero.
Source: Nature Microbiology  Volume: 7 – 7, pp. 1001 – 1015 Published: 2022
DOI:10.1038/s41564-022-01134-8

Transparent, UV-blocking, and high barrier cellulose-based bioplastics with naringin as active food packaging materials

Author(s):S. Guzman-Puyol; J. Hierrezuelo; J.J. Benítez; G. Tedeschi; J.M. Porras-Vázquez; A. Heredia; A. Athanassiou; D. Romero; J.A. Heredia-Guerrero.
Source: International Journal of Biological Macromolecules  Volume: 209, pp. 1985 – 1994 Published: 2022
DOI:10.1016/j.ijbiomac.2022.04.177

Chitin deacetylase, a novel target for the design of agricultural fungicides.

Author(s): J.M. Martínez-Cruz; Á. Polonio; R. Zanni; D. Romero; J. Gálvez; D. Fernández-Ortuño; A. Pérez-García.
Source: Journal of Fungi  Volume: 7 – 12 Published: 2021
DOI:10.3390/jof7121009

Chemical Proportionality within Molecular Networks

Author(s):D. Petras; A.M. Caraballo-Rodríguez; A.K. Jarmusch; C. Molina-Santiago; J.M. Gauglitz; E.C. Gentry; P. Belda-Ferre; D. Romero; S.M. Tsunoda; P.C. Dorrestein; M. Wang.
Source: Analytical Chemistry  Volume: 93 – 38 pp. 12833 – 12839 Published: 2021
DOI:10.1021/acs.analchem.1c01520

Chemical interplay and complementary adaptative strategies toggle bacterial antagonism and co-existence

Author(s): Carlos Molina-Santiago, David Vela-Corcía, Daniel Petras, Luís Díaz-Martínez, Alicia Isabel Pérez-Lorente, Sara Sopeña-Torres, John Pearson, Andrés Mauricio Caraballo-Rodríguez, Pieter C Dorrestein and Diego Romero
Source: Cell Reports  Volume: 36 (4) Published: July 2021
DOI:10.1016/j.celrep.2021.109449

Sporulation is dispensable for the vegetable‐associated life cycle of the human pathogen Bacillus cereus

Author(s): María Luisa Antequera-Gómez, Luís Díaz-Martínez, Juan Antonio Guadix, Ana María Sánchez-Tévar, Sara Sopeña-Torres, Jesús Hierrezuelo and Diego Romero
Source: Microbial Biotechnology  Volume: 14 (4) Published: July 2021
DOI: 10.1111/1751-7915.13816

Bacterial extracellular matrix as a natural source of biotechnologically multivalent materials

Author(s): Carlos Molina-Santiago, Antonio de Vicente and Diego Romero
Source: Computational and Structural Biotechnology Journal   Volume: 19  Published: January 2021
DOI: 10.1016/j.csbj.2021.05.008

Multifunctional Amyloids in the Biology of Gram-Positive Bacteria

Author(s): Ana Álvarez-Mena, Jesús Cámara-Almirón, Antonio de Vicente and Diego Romero
Source: Microorganisms    Volume: 8(12) Published: Dic 2020
DOI: doi.org/10.3390/microorganisms8122020

Dual functionality of the amyloid protein TasA in Bacillus physiology and fitness on the phylloplane

Author(s): Jesús Cámara-Almirón, Yurena Navarro, Luis Díaz-Martínez, María Concepción Magno-Pérez-Bryan, Carlos Molina-Santiago, John R. Pearson, Antonio de Vicente, Alejandro Pérez-García & Diego Romero.
Source: Nature Communications Number: 11 Published: AP 2020
DOI: 10.1038/s41467-020-15758-z

Two genomic regions encoding exopolysaccharide production systems have complementary functions in B. cereus multicellularity and host interaction

Author(s):  Joaquin Caro-Astorga, Ana Álvarez-Mena, Jesús Hierrezuelo, Juan Antonio Guadix, Zahira Heredia-Ponce, Yohanna Arboleda-estudillo, Elena González-Munoz, Antonio de Vicente & Diego Romero.
Source: Sci Rep
Volume:  10 Pages: (1):1000.
Published: Jan 2020
DOI: 10.1038/s41598-020-57970-3

Biofilm formation displays intrinsic offensive and defensive features of Bacillus cereus

The extracellular matrix protects Bacillus subtilis colonies from Pseudomonas invasion and modulates plant co-colonization.

Molecular architecture of bacterial amyloids in Bacillus biofilms.